SO2 testing is an integral part of winemaking and SO2 management begins as soon as you add sulfite to the wine. Having the right amount of SO2 in your wine prevents enzymatic browning and guards against premature oxidation. SO2 preserves freshness and color and it helps stabilize a wine against microbial spoilage. If a wine does not contain the required amount of SO2, chances are it won't gracefully make it past one year in the bottle (depending on the wine pH).
Bound & Free SO2:
There are two forms of SO2 that we need to be aware of: Bound SO2 and Free SO2. When you make an SO2 addition, a portion of it binds with elements in the must or wine (acetaldehyde, yeast, bacteria, sugars, tannin, oxygen, etc.) and is referred to as bound. The rest of the addition remains unbound and is referred to as free. It is this latter portion we are interested in because only this free SO2 brings you the previously mentioned benefits/protection.
How Much SO2 Should I Use?:
The actual amount of SO2 needed is quite small, and is referred to as "ppm" (parts per million). The goal with SO2 testing and management is to use the least amount needed in order to maintain a beneficial saturation level (0.8 ppm molecular of SO2), while trying to avoid adding too much, which would flaw the wine by giving it a sulfite smell and/or taste. Interestingly, the amount of SO2 needed to protect a wine is dependent on its pH; at a higher wine pH the required ppm of SO2 is higher than it is at a lower wine pH. For example, a wine with a pH of 3.3 requires 27 ppm free SO2, while a wine with a pH of 3.7 needs 64 ppm free SO2 to remain protected.
Complete information on SO2 management, including the pH/SO2 amount needed chart can be found in our Red Winemaking Manual and our White Winemaking Manual.
SO2 Testing
-
#5 Rubber Stopper - 2 holes
Rubber stopper for use with the MT140 Economy Aeration-Oxidation System. Top Outside Diameter (OD) 27mm Bottom Outside Diameter (OD) 23mm Height 25mm
$5.99
-
Hanna Calibration Solution for Titrator - 120mL (Hanna# HI 84500-55)
The HI84500-55 is a 120 mL bottle of pump calibration standard that is used to calibrate the dosing system of the HI84500 Sulfur Dioxide Mini Titrator for Wine Analysis. A calibrated dosing pump allows for the accurate determination of the amount of titrant used when performing a titration. The amount of volume dosed along with the concentration of the titrant is used by the pre-programmed formula within the HI84500 in determining the exact concentration of sulfur dioxide. The pump calibration procedure should be performed anytime the titrant or ORP electrode is replaced. Standardized solution Clearly marked expiration date and lot number Air tight bottle with tamper-proof seal to ensure quality Analysis of sulfur dioxide in wine, as performed on the HI84500 mini titrator, utilizes a simple sample preparation, a high quality titrant dosing pump, potentiometric endpoint detector, and automatic computations. To maintain the high precision of the titrator, a simple pump calibration procedure is performed with HI84500-55 pump calibration solution. The pump calibration procedure involves the analysis of a known volume of a known solution. The pump calibration procedure should be performed anytime the titrant, syringe, tubing or ORP electrode is replaced. Periodic calibrations should be performed in order to accurately determine the free and total sulfur dioxide levels in wine. The HI84500 determines the free and total sulfur dioxide concentration in wine using the Ripper method. Excess iodide is added to the wine sample and reacts with the iodate titrant to produce iodine: IO3- + 5I- + 6H+ → 3I2 + 3H2O The iodine produced reacts with sulfur dioxide in the wine according to the redox reaction below: H2SO3 + I2 → H2SO4 + 2HI Replacement reagents required to operate the HI84500 include: Low Range - 1.0 to 40.0 ppm SO2 High Range - 30 to 400 ppm SO2 Pump calibration standard HI84500-55 HI84500-55 Titrant solution HI84500-50 HI84500-51 Acid reagent HI84500-60 HI84500-60 Alkaline reagent (Total SO2) HI84500-61 HI84500-61 Stabilizer powder packets HI84500-62 HI84500-62
$49.99
-
1/4 in. Plastic Push Together Quick Disconnect (QD) Assembly
Not for use under pressure. Can be used for gas tubing like in our MT140 SO2 Test Kit or for splicing racking tubing up to 3/8" ID.
$7.99
-
Hanna Alkaline Solution for Titrator - 120mL (Hanna# HI84500-61)
The HI84500-61 is a 120 mL bottle of alkaline reagent required for determination of total sulfur dioxide in wine using the HI84500 Sulfur Dioxide Mini Titrator for Wine Analysis. This alkaline reagent is used to raise the pH value of the sample prior to performing a total sulfur dioxide titration. With 5 mL of reagent required per total SO2 test, there is enough alkaline reagent for approximate 24 tests. Pre-measured solution Clearly marked expiration date and lot number Air tight bottle with tamper-proof seal to ensure quality The HI84500-61 alkaline reagent is necessary for the analysis of total sulfur dioxide. The alkaline reagent raises the pH and liberates bound sulfur dioxide. This pH adjustment ensures that all of the free and bound sulfur dioxide is accounted for to give an accurate measurement of total sulfur dioxide. The HI3148B ORP electrode is used to detect the endpoint for both the low (1.0 to 40.0 ppm of SO2) and high (30 to 400 ppm of SO2) ranges. The HI84500 can be used with all wines, including red, which are difficult to test using traditional methods due to the difficulty to observe a distinctive color change when the endpoint is reached. The HI84500 determines the free and total sulfur dioxide concentration in wine using the Ripper method. Excess iodide is added to the wine sample and reacts with the iodate titrant to produce iodine: IO3- + 5I- + 6H+ → 3I2 + 3H2O The iodine produced reacts with sulfur dioxide in the wine according to the redox reaction below: H2SO3 + I2 → H2SO4 + 2HI Replacement reagents required to operate the HI84500 include: Low Range - 1.0 to 40.0 ppm SO2 High Range - 30 to 400 ppm SO2 Pump calibration standard HI84500-55 HI84500-55 Titrant solution HI84500-50 HI84500-51 Acid reagent HI84500-60 HI84500-60 Alkaline reagent (Total SO2) HI84500-61 HI84500-61 Stabilizer powder packets HI84500-62 HI84500-62 Requires only 5mL per test. Pre-measured solution with clearly marked expiration date and lot numbers in a tamper proof bottle, can be utilized for all wines including red.
$28.00
-
Erlenmeyer Flask | 125mL | Narrow Neck
Made with durable borosilicate 3.3 glass, these Erlenmeyer flasks are suitable for most applications. They feature a heavy-duty beaded rim to help prevent breakage and have easy-to-read graduations. Able to withstand temperatures of -50 to 550 °C (-58 to 1022 °F), they are autoclavable for sterilization. In the lab world, these flasks are known as "Student Grade". Student grade is not the highest grade flasks and will have minor defects. Please note this 125mL flask has a narrow neck compared to our Y402 unit and will not accept a #5 stopper. This unit will need a #3 stopper. Specs: Material: Borosilicate Glass Capacity (mL): 125 Capacity (oz): 4 Min Graduation (mL): 50 Max Graduation (mL): 125 Graduation Interval (mL): 20 Overall Height (in): 4 59/64 Overall Height (cm): 12.5 Top Outside Diameter (mm): 22 Autoclavable: Yes Base Diameter (cm): 7.2 Inside Rim Diameter (mm): 20
$9.99 $5.99
