SO2 Testing

The Ultimate Winemaker’s Companion! Includes everything you need to analyze SO2, pH, and TA in one kit Tests take less than 2 minutes to perform and are incredibly accurate with pH readings to 1/100 of a point Combines the SO2 detection of the SC-100A and the pH/TA analysis of the SC-200 into one compact instrument Audible buzzer and indicator light alert you to when the test is complete Affordable wine analysis averaging roughly $1 per test One lean, mean wine analyzing machine. The SC-300 combines the SO2 detection of the SC-100A and the pH/TA analysis of the SC-200 into one power-packed instrument. It’s built on the same hardware and software as our other two analyzers, so the accuracy is superb. And it’s just as easy to use – The audible buzzer and visual stop light will automatically let you know when you’re tests are done! Because it does not rely on a color change, the SC-300 eliminates the guesswork in Ripper sulfite tests, especially in red wines! Why Choose Vinmetrica’s SC-300? Where else will you find a single, compact instrument that can measure sulfite (SO2), pH and TA (Titratable Acidity) levels throughout the fermentation and aging process? Even in Red Wines! It’s Accurate: SO2: ± 2 ppm Free SO2. TA: ± 0.2 g/L Tartaric acid pH: better than ± 0.02 pH units Quick! – Less than 2 minutes per test! Affordable – About a $1 per test!* Easy to Use – No mystery solutions, pumps or tubing. *SO2 (Sulfite) Tests cost approximately 70¢ / Test (the cost of the reagents per test). If you do a combined pH and TA analysis, the cost of reagents for the pH/TA test is approximately $1.12 / Test. Complete Wine Testing Kit – Includes Everything Needed to Analyze Sulfite (SO2), pH and TA (Titratable Acidity) levels in Your Wines & Musts: SO2 reagents pH/TA reagents Two titration beakers Two 5 mL syringes Two transfer pipettes SO2 electrode pH electrode 25 mL sampling pipette (for SO2) 5 mL sampling pipette (for pH/TA) The SC-300 Operating Manual The SC-300 analyzer gives accurate SO2, pH and TA values. Kit includes everything to perform about 50 sulfite tests and 30 TA tests: both electrodes, all reagents, pipettes, syringe and beaker. A Quick Overview – Wine Analysis Procedures with the SC-300: Easy SO2 Test Attach SO2 electrode, press the power button, change to SO2 mode and press enter. Draw up 5 mL of titrant into the syringe and place 25 mL of wine in beaker. Add ~2 mL of the acidifier and ~2 mL of the reactant to wine. Place and hold the SO2 electrode in the wine sample and swirl beaker. Add titrant from syringe dropwise until 15 second long endpoint signal reached. Multiply the titration volume by 20, the SO2 conversion factor, results in ppm Free SO2. Simple pH Test Attach the pH electrode, set aside storage solution, rinse with DI water and blot gently with a paper towel. Press the power button then press the ‘Mode’ button and switch the SC-200 into ‘Cal’ mode. Calibrate using the pH 4 and pH 7 reference standards provided. Put the SC-200 in pH mode. Take 5 mL sample of wine, place in a beaker. Take a pH reading. Accurate TA Test For TA test, withdraw 5 mL of the TA Titrant with the syringe. Take 5 mL wine sample and add ~15 mL of deionized water. Put SC-200 into TA mode, place clean pH electrode gently in solution. Add TA Titrant from the syringe dropwise until the endpoint is reached indicated with the visual and audible signals. Record the volume (in mL) of titrant used. Multiply the titrantion volume by 2, the TA conversion factor, results in g/L Tartaric Acid. Vinmetrica Troubleshooting Guide

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The SC-100A puts you in control of your wine sulfite analysis. It’s highly accurate and sensitive titration system conveniently alerts you visually and audibly when the endpoint is detected by its electrode. Because it doesn’t rely on a color change, the SC-100A eliminates the guesswork in Ripper sulfite tests, especially in red wines! The SC-100A analyzer gives you confidence to run your sulfite assays accurately and quickly. Kit includes everything to perform approx. 50 tests: electrode, reagents, pipettes, syringe and beaker. How It Works: The SC-100A improves on the tried and true Ripper Method by eliminating the guesswork in colorimetric analysis and replacing it with digital detection indicators. It uses an amperometric electrode and replaces the iodine reagent with a more stable reagent. Its ease of use and accurate amperometric titration method vastly improves on the original Ripper Method. The SO2 Titrant reacts with the sulfite in the wine. When all the free SO2 is titrated, the endpoint is signalled by visual and audible indicators. The test endpoint is much more sensitive than the starch endpoint commonly employed for sulfite titrations. It’s sharp and clear, even when titrating red wines and musts. Once the syringe titration is complete, determine the titration volume (which is the difference of titrant you started with and its final reading on the syringe) and multiply it by 20 (the conversion factor). The conversion factor takes into account the known concentration of the titrant and some simple unit conversions to determine the ppm Free SO2 in your wine sample. So for example if you used 1.2 mL of SO2 Titrant during the titration, your equation would look like this: 1.2 mL X 20 = 24 ppm Free SO2 Simple To Use Attach SO2 electrode, press the power button, change to ‘SO2′ mode and press enter. Draw up 5 mL of titrant into the syringe. Then place 25 mL of wine in beaker. Add ~2 mL of the acidifier and ~2 mL of the reactant to wine. Place and hold the SO2 electrode into the wine sample and swirl beaker. Add titrant dropwise, pause when device beeps, until 15 seconds of beeps reached. Multiply the titration volume by 20, the SO2 conversion factor, results in ppm Free SO2. Complete Kit Includes Everything You Need for Your First 50 Tests! SO2 reagents Titration beaker 5 mL syringe SO2 electrode 25 mL sampling pipette 2 transfer pipettes Vinmetrica Troubleshooting Guide

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The SC-300 Pro Kit has everything the normal SC-300 has plus these accessories: The Lab Support Stand Vinmetrica Electrode Holder The Double Burette Clamp Magnetic Stirrer Rinse Bottle A Small Tube of Burette Detergent 25 mL Glass Burette. The Ultimate Winemaker’s Companion shipped free from MoreWine! One lean, mean wine analyzing machine. The SC-300 combines the SO2 detection of the SC-100A and the pH/TA analysis of the SC-200 into one power-packed instrument. It’s built on the same hardware and software as our other two analyzers, so the accuracy is superb. And it’s just as easy to use – The audible buzzer and visual stop light will automatically let you know when you’re tests are done! Because it does not rely on a color change, the SC-300 eliminates the guesswork in Ripper sulfite tests, especially in red wines! Why Choose Vinmetrica’s SC-300? Where else will you find a single, compact instrument that can measure sulfite (SO2), pH and TA (Titratable Acidity) levels throughout the fermentation and aging process? Even in Red Wines! It’s Accurate: SO2: ± 2 ppm Free SO2. TA: ± 0.2 g/L Tartaric acid pH: better than ± 0.02 pH units Quick! – Less than 2 minutes per test! Affordable – About a $1 per test!* Easy to Use – No mystery solutions, pumps or tubing. *SO2 (Sulfite) Tests cost approximately 70¢ / Test (the cost of the reagents per test). If you do a combined pH and TA analysis, the cost of reagents for the pH/TA test is approximately $1.12 / Test. Complete Wine Testing Kit – Includes Everything Needed to Analyze Sulfite (SO2), pH and TA (Titratable Acidity) levels in Your Wines & Musts: SO2 reagents pH/TA reagents Two titration beakers Two 5 mL syringes Two transfer pipettes SO2 electrode pH electrode 25 mL sampling pipette (for SO2) 5 mL sampling pipette (for pH/TA) The SC-300 Operating Manual The SC-300 analyzer gives accurate SO2, pH and TA values. Kit includes everything to perform about 50 sulfite tests and 30 TA tests: both electrodes, all reagents, pipettes, syringe and beaker. A Quick Overview – Wine Analysis Procedures with the SC-300: Easy SO2 Test Attach SO2 electrode, press the power button, change to SO2 mode and press enter. Draw up 5 mL of titrant into the syringe and place 25 mL of wine in beaker. Add ~2 mL of the acidifier and ~2 mL of the reactant to wine. Place and hold the SO2 electrode in the wine sample and swirl beaker. Add titrant from syringe dropwise until 15 second long endpoint signal reached. Multiply the titration volume by 20, the SO2 conversion factor, results in ppm Free SO2. Simple pH Test Attach the pH electrode, set aside storage solution, rinse with DI water and blot gently with a paper towel. Press the power button then press the ‘Mode’ button and switch the SC-200 into ‘Cal’ mode. Calibrate using the pH 4 and pH 7 reference standards provided. Put the SC-200 in pH mode. Take 5 mL sample of wine, place in a beaker. Take a pH reading. Accurate TA Test For TA test, withdraw 5 mL of the TA Titrant with the syringe. Take 5 mL wine sample and add ~15 mL of deionized water. Put SC-200 into TA mode, place clean pH electrode gently in solution. Add TA Titrant from the syringe dropwise until the endpoint is reached indicated with the visual and audible signals. Record the volume (in mL) of titrant used. Multiply the titrantion volume by 2, the TA conversion factor, results in g/L Tartaric Acid. Vinmetrica Troubleshooting Guide

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For determination of Total SO2 (not included in SC-100A or SC-300 Analyzer kits). Guaranteed for 6 months. To avoid cross-contamination with other reagents, use a dedicated syringe or burette to dispense the NaOH reagent. Store reagents in a dark, cool place with the bottle tightly sealed to maximize shelf life. Good for about 12 tests.

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The HI84500-60 is a 230 mL bottle of acid reagent required for both low and high range determinations of sulfur dioxide in wine using the HI84500 Sulfur Dioxide Mini Titrator for Wine Analysis. The acid reagent is used to lower the pH value of the sample prior to performing a titration. With 5 mL of reagent required per test, there is enough acid reagent for approximately 46 tests. Pre-measured solution Clearly marked expiration date and lot number Air tight bottle with tamper-proof seal to ensure quality The HI84500-60 acid reagent is necessary to lower the pH of the wine sample before a titration can be performed for either free or total sulfur dioxide. The adjustment of the wine sample with 5 mL of the acidic reagent ensures the sample is at the correct pH. The addition of HI84500-62 stabilizing powder is also added prior to performing the titration. The HI3148B ORP electrode is used to detect the endpoint for both the low (1.0 to 40.0 ppm of SO2) and high (30 to 400 ppm of SO2) ranges. The HI84500 can be used with all wines, including red, which are difficult to test using traditional methods due to the difficulty to observe a distinctive color change when the endpoint is reached.   The HI84500 determines the free and total sulfur dioxide concentration in wine using the Ripper method. Excess iodide is added to the wine sample and reacts with the iodate titrant to produce iodine:   IO3- + 5I- + 6H+ → 3I2 + 3H2O   The iodine produced reacts with sulfur dioxide in the wine according to the redox reaction below:   H2SO3 + I2 → H2SO4 + 2HI   Replacement reagents required to operate the HI84500 include:     Low Range - 1.0 to 40.0 ppm SO2 High Range - 30 to 400 ppm SO2 Pump calibration standard HI84500-55 HI84500-55 Titrant solution HI84500-50 HI84500-51 Acid reagent HI84500-60 HI84500-60 Alkaline reagent (Total SO2) HI84500-61 HI84500-61 Stabilizer powder packets HI84500-62 HI84500-62 Requires only 5mL per test Pre-measured solution with clearly marked expiration date and lot numbers in a tamper proof bottle, can be utilized for all wines including red.    

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